منابع مشابه
Distribution of ultraviolet-induced lesions in simian virus 40 DNA.
In order to analyze the molecular mechanisms of mutagenesis in mammalian cells, we devised an analytical assay using Simian Virus 40 as biological probe. To study the possible correlations between the distribution of the lesions on the treated DNA and the distribution of mutations, we have located and quantified the lesions induced by ultraviolet light (254 nm) on a SV40 DNA fragment. At a flue...
متن کاملRole of DNA polymerase alpha and DNA primase in simian virus 40 DNA replication in vitro.
The role of DNA polymerase alpha (pol alpha) and DNA primase has been investigated in the simian virus 40 (SV40) DNA replication system in vitro. Removal of pol alpha and primase activities from crude extracts of HeLa cells or monkey cells by use of an anti-pol alpha immunoaffinity column resulted in the loss of replication activity. The addition of purified pol alpha-primase complex isolated f...
متن کاملPurification of DNA polymerase delta as an essential simian virus 40 DNA replication factor.
DNA replication from the SV40 origin can be reconstituted in vitro using purified SV40 large T antigen, cellular topoisomerases I and II, replication factor A (RF-A), proliferating cell nuclear antigen (PCNA), replication factor C (RF-C), and a phosphocellulose fraction (IIA) made from human cell extracts (S100). Fraction IIA contains all DNA polymerase activity required for replication in vitr...
متن کاملSimian virus 40 integration site in an adenovirus 7-simian virus 40 hybrid DNA molecule.
The E46(+) strain of Adenovirus 7 is a mixed-virus population containing defective Adenoviurs 7-SV40 hybrid particles and helper, nonhybrid Adenovirus 7 particles. We have applied electron microscopic mapping techniques to obtain a physical map of the genome of the hybrid particles present in E46(+)PL1, a substrain of E46(+) derived from a single two-hit plaque. DNA molecules extracted from pur...
متن کاملDNA synthesis by partially purified replicating simian virus 40 chromosomes.
We have partially purified replicating simian virus 40 (SV40) chromosomes in a form which allows continued DNA synthesis in vitro. We first prepare a soluble DNA-synthesizing system from SV40-infected monkey cells and then sediment the components through a neutral sucrose gradient of extremely low ionic strength. Replicating SV40 chromosomes isolated from such gradients are capable of continuin...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1967
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-1-2-163